Hematological and genotoxic effects of copper (II) chloride on Oncorhynchus mykiss

Oncorhynchus mykiss is amongst the most important species in internal water fish breeding. O. mykiss having very high economical value is largely used in acute toxicity tests. The target of this study is to elucidate the genotoxic effect of CuCl2 upon O. mykiss. The bio assay was carried out in triplicate. The 96h LC50 value was found to be 0.48 mg/L (0.43-0.54 mg/L) from the literature and the sublethal concentration was taken as 0.048 mg/L as the 1/10 of this value. The blood samples taken for the determination hematocrit level were centrifuged at 11500 rpm for 4 minutes. Micronucleus test (MN) was carried out using blood preparations obtained. The mean MN values of the experimental group were found to be higher than those of control group. The hematocrit level of the control and the experimental groups were found to be 29.91 % and 25.01 % respectively. The study reveals that the MN frequencies of O. mykiss species in the experimental group was observed to be higher than those of experimental group while the hematocrit level of the control group was found to be higher than the experiential group. These data clearly show that the CuCl2 has a high level toxic effect upon O. mykiss.

Bakýr (II) Klorür’ün Oncorhynchus mykiss üzerindeki hematolojik ve genotoksik etkileri

Oncorhynchus mykiss, iç su balýkçýlýðýnýn en önemli türleri arasýndadýr. Ekonomik deðeri yüksek olan O. mykiss akut toksisite testlerinde yaygýn olarak kullanýlmaktadýr. Bu çalýþmada Bakýr (II) Klorür’ün (CuCl2) O. mykiss üzerindeki genotoksik etkisinin belirlenmesi amaçlanmýþtýr. Biyodeney üç tekrarlý yapýlmýþtýr. Akut toksisite deneyinde 96 saatlik LC50 deðeri olarak literatür bilgisine dayalý 0,48 mg/L’nin (0,43-0,54 mg/L) 1/10’u 0,048 mg/L sublethal konsantrasyon olarak kullanýlmýþtýr. Deney bitiminde hematokrit düzeyi için kan örnekleri 11500 rpm’de 4 dakika santrifüj edilmiþtir. Preparat hazýrlanmýþ ve mikronükleus (MN) testi yapýlmýþtýr. Deney grubunda MN ortalama deðerleri kontrol grubuna göre yüksek olarak bulunmuþtur. Kan örneklerinde hematokrit seviyesi kontrol grubunda ortalama % 29,91 iken deney grubunda % 25,01 olarak belirlenmiþtir. Bu çalýþma sonucunda; deney grubundaki O. mykiss bireylerinin eritrositlerindeki MN frekansýnýn kontrol grubuna göre yüksek olduðu, hematokrit seviyesinin ise kontrol grubuna göre deney grubunda düþtüðü anlaþýlmýþtýr. Bu sonuçlar Bakýr (II) Klorür’ün yüksek düzeyde toksik etkisi olduðunu göstermektedir.